biorad dcode apparatus dgge  (Bio-Rad)

Journal: Aquatic Microbial Ecology

Article Title: Structure of bacterial communities in aquatic systems as revealed by filter PCR

doi: 10.3354/ame026013

Figure Lengend Snippet: Fig. 2. DGGE gel of amplicons from PCR with isolated DNA and from filter PCR. The primers were GC358F and 907RM, which are specific for a portion of the 16S rRNA gene. The volume of the PCR reaction loaded in all sample lanes was the same. Arrow indicates a band found in the isolated DNA lane that is not obvious in the filter PCR lanes. Samples taken in November 1999

Article Snippet: Electrophoresis was performed at 60°C for 20 h at 100 V using a BioRad Dcode DGGE apparatus.

Techniques: Isolation

Journal: Aquatic Microbial Ecology

Article Title: Structure of bacterial communities in aquatic systems as revealed by filter PCR

doi: 10.3354/ame026013

Figure Lengend Snippet: Fig. 3. DGGE gel of amplicons from PCR with isolated DNA and from filter PCR. The primers were GC358F and 517R for a portion of the 16S rRNA gene. Standards (STD) were ampli- cons from 3 bacterial isolates. The volume of the PCR reaction loaded in all sample lanes was the same. Samples taken in May 2000

Article Snippet: Electrophoresis was performed at 60°C for 20 h at 100 V using a BioRad Dcode DGGE apparatus.

Techniques: Isolation

Journal: Aquatic Microbial Ecology

Article Title: Structure of bacterial communities in aquatic systems as revealed by filter PCR

doi: 10.3354/ame026013

Figure Lengend Snippet: Fig. 4. DGGE gel of 16S rRNA genes from pure bacterial isolates analyzed either separately or in mixtures. Isolates are given in Table 1. Mixtures consisted of: (1) the strains mixed together, fil- tered, and then subjected to filter PCR (F); (2) isolated DNA from each strain was mixed and then used a template in normal PCR (I); and (3) amplicons from the bacterial isolates were mixed after PCR in order to test the resolving power of the DGGE gel (C). Arrow indi- cates a band observed in the isolated DNA mixture that is not obvious in the mixed strain filter PCR lane

Article Snippet: Electrophoresis was performed at 60°C for 20 h at 100 V using a BioRad Dcode DGGE apparatus.

Techniques: Isolation